Carteolol Hydrochloride Suppresses the Generation of Reactive Oxygen Species and Rescues Cell Death After Ultraviolet Irradiation of Cultured Lens Epithelial Cells
Yuichi Kaji*, Takahiro Kiuchi , Tetsuro Oshika
Identifiers and Pagination:Year: 2010
First Page: 60
Last Page: 65
Publisher Id: TOOPHTJ-4-60
Article History:Received Date: 22/6/2010
Revision Received Date: 13/8/2010
Acceptance Date: 17/8/2010
Electronic publication date: 12/10/2010
Collection year: 2010
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Anti-oxidant activities of adrenergic β-blockers are proposed in various organs. The aim of the present study was to investigate the effect of carteolol hydrochloride, an adrenergic β-blocker, on the production of reactive oxygen species (ROS) and the viable cell number after ultraviolet irradiation of cultured lens epithelial cells (LECs).
Materials and Methodology:
Cultured LECs were exposed to 0, 10–5, 10–4, and 10–3 M carteolol hydrochloride for 30 min followed by ultraviolet B (UVB) irradiation at intensity of 100, 200, or 400 mJ/cm2. The amount of ROS in the LECs was measured using dichlorodihydrofluorescein at 30 min after exposure to UVB. In addition, the number of living LECs was counted at 15 h after exposure to UVB.
Exposure to 10–3 M carteolol hydrochloride significantly decreased the amount of ROS after exposure to UVB at intensities of 100, 200, and 400 mJ/cm2. In addition, 10–3 M carteolol hydrochloride significantly increased the viable cell number after exposure to UVB at 400 mJ/cm2. However, 10–4 and 10–5M carteolol hydrochloride had no significant effect on ROS or the viable cell number in LECs.
Carteolol hydrochloride protects LECs against UVB irradiation by inhibiting the intracellular production of ROS.
Various population-based studies have clearly shown that the prevalence of glaucoma increases with age [1, 2]. This means that considerable numbers of patients with glaucoma also have cataracts or have already received cataract surgery. In addition, long-term topical application of anti-glaucoma drugs is needed in most of the patients with glaucoma. At present, however, little is known about the effect of anti-glaucoma drugs on cataract formation. Accordingly, the effect of these drugs on the development of cataracts should be investigated.
β-Adrenergic blockers are widely used to treat glaucoma and ocular hypertension as well as hypertension and cardiac arrhythmia. Various papers have shown that β-adrenergic blockers serve as an antioxidant in experimental models. For example, antioxidant effects are reported for timolol, betaxolol, and niparadiol on cultured retinal ganglion cells ; for metipranolol and desacetyl metipranolol on the brain ; and for arotinolol on muscles . However, the minute molecular mechanisms of antioxidant activities of β-adrenergic blockers are still unclear. Among the various β-adrenergic blockers, molecular mechanism of antioxidant activity of carteolol hydrochloride (5-(3-tert-butylamino-2-hydroxy)propoxy-3,4-dihydro-2(1H)-quinolinone monohydro- chloride) is partially revealed [6, 7]. Carteolol hydrochloride is one of the β-adrenergic blockers and widely used to treat cardiac arrhythmia, glaucoma, and ocular hypertension [8, 9]. The reported antioxidant properties of carteolol hydrochloride have, however, differed among the previous studies. Tanito et al. have reported that carteolol hydrochloride has antioxidant properties in the cornea and retina [6, 10], but not in retinal ganglion cells, the brain, or muscles [3, 5]. This indicates that the antioxidant properties of this chemical depend on the type of cell and the manner in which oxidative stress is induced. For this reason, the antioxidant activities of carteolol hydrochloride should be examined in various ocular tissues.
In the present study, we attempted to reveal the effect of carteolol hydrochloride on cataract formation. However, it is practically difficult to evaluate the effect of the topical application of carteolol hydrochloride on cataract formation over the long term (years) using in vivo models. Recently, ultraviolet irradiation of cultured lens epithelial cells (LECs) has been used as an in vitro model of cataract formation . Ultraviolet light is one of the important accelerators of cataract formation [12, 13]. In addition, ultraviolet irradiation of cultured LECs in vitro is known to induce various changes, such as racemization and glycation of crystalline molecules, apoptosis, activation of p53, and induction of reactive oxygen species (ROS) , that are closely related to the development of cataracts in vivo. For these reasons, we examined the effect of carteolol hydrochloride on the number of living cells and intracellular ROS after ultraviolet irradiation of cultured lens epithelial cells.
MATERIALS AND METHODOLOGY
Human lens epithelial cells (LECs), line SRA01/04, were purchased from the RIKEN Cell Bank (Tsukuba, Ibaraki, Japan). The LECs were cultured in 60-mm culture dishes (Falcon, Lincoln Park, NJ) in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 15% fetal bovine serum (FBS), l-glutamine (2 mM), penicillin (100 units/mL), and streptomycin (0.1 mg/mL) in a humidified atmosphere containing 5% CO2 at 37 °C. After the LECs had reached confluence, a portion was subcultured in a 96-well culture plate (Falcon).
Carteolol hydrochloride was obtained from the Tokushima Research Institute, Otsuka Pharmaceutical Co., Ltd. 2′,7′-Dichlorodihydrofluorescein diacetate were purchased from Sigma.
Reactive Oxygen Species After Exposure to UVB
The LECs were seeded at 10,000 cells/well in96-well plates. Twelve hours after seeding, non-adherent LECs were washed away with the cultured medium. Carteolol hydrochloride was added to the culture medium at final concentrations of 0, 10–5, 10–4, and 10–3M and left for 30 min. Each well was then washed three times with phosphate-buffered saline (pH 7.4) (PBS) followed by the addition of 100 µl of culture medium without carteolol hydrochloride. The cells were exposed to ultraviolet B (UVB) (0, 100, 200, or 400 mJ/cm2 at an exposure time of 45 seconds per 100 mJ/cm2) using a UV transilluminator (Model UVM-57, with a peak at 302 nm; UVP, Inc., Upland, CA). The intensity of UVB was measured using a UV radiometer (SUV-T; Toray Techno Co., Japan) at the bottom of the culture plate. The UV-irradiated LECs were treated with 10 µM of 2′,7′-dichlorodihydrofluorescein diacetate in PBS for 30 min. Thereafter, the medium was discarded and the cells were washed with PBS. The fluorescence intensity was determined using a Varioskan microplate reader (Thermo Electric Corporation, Vantaa, Finland) at excitation and emission wavelengths of 485 nm and 535 nm, respectively. UVB-induced intracellular ROS were also confirmed using a fluorescence microscope (ECLIPSE 80i; Nikon, Tokyo, Japan) equipped with a cooled CCD camera (DXM1200C; Nikon).
Cell Viability Measurement After Exposure to UVB
Pretreatment with carteolol hydrochloride and UVB irradiation was performed as described above. Fifteen hours after the UVB irradiation, non-viable cells were removed by washing three times with PBS. Then the attached cells were removed by exposing each well to 0.25 mL of trypsin-EDTA (GIBCO, Grand Island, NY) for 20 minutes at 37 ºC. Isoton solution (American Scientific Products, McGraw, IL) was added to the cell suspension to a final volume of 10 mL, and the cell number of each well was counted with a Coulter counter (type Z1). The experiments were repeated 8 times in each condition.
The intensity of fluorescence and the cell viability are expressed as the mean ± SD. The sample size for each group was 16, and we repeated each experiment. The data for all test samples were analyzed using a one-way ANOVA and compared using the Dunnett test. A P value of <0.05 was considered statistically significant.
Visualization of the Amounts of Reactive Oxygen Species in Lens Epithelial Cells
Fig. (1) shows an example of the fluorescence in LECs treated with 2′,7′-dichlorodihydrofluorescein diacetate after 200 mJ/cm2 of UVB irradiation. Without exposure to UVB, the LECs exhibited almost no fluorescence. In contrast, intense fluorescence was observed in the cytoplasm and nuclei of the LECs after exposure to UVB. The intensity of fluorescence was reduced in the LECs pretreated with 10–3 M carteolol hydrochloride. All the images were taken with the same exposure time (20 msec) and visualized identically.
Quantification of Reactive Oxygen Species in Lens Epithelial Cells After Exposure to Ultraviolet Light
Fig. (2A) shows the intensity of fluorescence in the LECs treated with 2′,7′-dichlorodihydrofluorescein diacetate after exposure to UVB. The intensity of fluorescence was increased by the UVB irradiation in a dose-dependent manner. Fig. (2B-D) show the effect of carteolol hydrochloride on the intensity of fluorescence after exposure to 100, 200, and 400 mJ/cm2 of UVB, respectively. The intensity of fluorescence decreased with an increase in carteolol hydrochloride concentration. In addition, the intensity of fluorescence decreased significantly (P < 0.01) in cells treated with 10–3 M carteolol hydrochloride.
Viability of Lens Epithelial Cells After Exposure to Ultraviolet Light
Fig. (3A) shows the number of viable cells after exposure to UVB. Irradiation with UVB decreased the number of viable LECs significantly in a dose-dependent manner. Fig. (3B-D) show the effect of carteolol hydrochloride on the number of viable cells after exposure to 100, 200, and 400 mJ/cm2 of UVB, respectively. No significant effect was observed on the number of viable cells after exposure to 100 mJ/cm2 of UVB irradiation (Fig. 3B). In contrast, the number of viable LECs increased significantly when these cells were pretreated with 10–3 M carteolol hydrochloride and received 200 and 400 mJ/cm2 of UVB irradiation (Fig. 3C, 3D). Pretreatment with carteolol hydrochloride at concentrations of 10–4 and 10–5 had no significant effect on the viability of LECs that had received 200 or 400 mJ/cm2 of UVB irradiation.
The purpose of the present study was to investigate the antioxidant properties of carteolol hydrochloride on LECs after irradiation with ultraviolet light. The results of this study showed that carteolol hydrochloride (10–3 M) had protective effects on the LECs (increased the number of viable cells) by inhibiting the production of ROS. These results indicate that carteolol hydrochloride may have a protective effect with regard to the development of cataracts because the production of ROS after UV irradiation is one of the causes of cataract formation.
All types of eye drop medication might have an effect on the eye lens in vivo. The potential effect of anti-glaucoma eye drops on lens epithelial cells is of particular importance because these products are used for periods extending over years in patients with glaucoma. Among various anti-glaucoma drugs, prostaglandin analogues are reported to have effects on cataract formation. Andley et al., for example, have reported that prostaglandin E2 suppresses and prostaglandin F2 accelerates UV-induced cataract formation . On the basis of the results of this study, these authors promoted awareness that the topical application of PGF2 analogues may induce cataracts. In addition, the authors reported that increased production of prostaglandins is involved in the development of UV-induced cataracts. Indeed, UV irradiation of cultured LECs increased the production of prostaglandins by activating cyclooxygenase [16, 17]. For these reasons, we should be aware of the potential risks of the long-term use of anti-glaucoma eye drops containing prostaglandin analogues and β-adrenergic blockers.
Oxidative stresses are closely related to the development of cataracts. Loss of reduced glutathione  and increases in oxidized residues in proteins  and lipid peroxidation [20, 21] are observed in human cataracts. Among various ROS, H2O2 is the major oxidant contributing to cataract formation . Elevated H2O2 concentrations are observed in both the lens and aqueous humor of the eyes of patients with cataracts [21, 22]. In addition, lenses cultured in medium containing elevated H2O2 develop cataracts. Furthermore, H2O2 can elicit protein modifications, such as glycation, oxidation, and cross-linking, that are observed in human lenses with cataracts . Among the various methods for evaluation of RPS in cells, we have used a fluorescent probe 2′,7′-dichlorodihydrofluorescein diacetate  for three reasons. First, the compound is commercially available and widely used to evaluate the intracellular concentration of ROS. Second, this compound reflects the intracellular concentration of various ROS including H2O2, NO, OH, and ONOO-. Third, not only the concentration of ROS, but also intracellular localization of ROS can be visualized under a fluorescent microscope. In the present study, a significant increase in 2′,7′-dichlorofluorescein fluorescence and a decrease in cell viability were observed after UV exposure. These changes were inhibited by the addition of carteolol hydrochloride in a dose-dependent manner. The results indicate that carteolol hydrochloride would inhibit cataract formation in an in vitro model.
Although apparently promising, the results of the present study do not necessarily suggest that topical application of carteolol hydrochloride inhibits cataract formation, due to the following reasons. First, we analyzed ROS production and cell death after UV irradiation in cultured LECs in vitro, which is not identical to cataract formation in vivo. However, we do believe that the results of the present study provide indirect evidence indicating that carteolol hydrochloride would have protective effects against cataract formation because UV irradiation and ROS production are important factors in cataract development [25, 26].
Second, although carteolol hydrochloride at a concentration of 10–3 M inhibited ROS generation and protected against cell death after UV exposure, this concentration is considerably higher than that resulting from the topical application of carteolol hydrochloride. Fujio et al. have reported that the maximum concentration of carteolol hydrochloride in the aqueous humor after topical application is 806 ± 147 ng/mL (2.46 ± 0.45 mM) . However, the concentration of carteolol hydrochloride in the lens should be much smaller than that of aqueous humor although the correct data are not reported. Accordingly, topically applied carteolol hydrochloride could not reach a concentration of 10–3M in the lens. Nevertheless, carteolol hydrochloride exhibited a dose-dependent antioxidant effect, although the effect was not statistically significant at concentrations of 10–4 and 10–5 M.
Third, the dosage of UBV irradiation was 100-400 mJ/cm2, which was none physiological. However, the intensity of UVB irradiation at 100-400 mJ/cm2 can be used in evaluation of both the production of reactive oxygen species and the cell death in in vitro studies. For example, Andley et al. evaluated the DNA repair and the cell survival of cultured lens epithelial cells after 400 mJ/cm2 of UBV irradiation . Yao et al. have analyzed the reactive oxygen species after UBV irradiation at 0-90 mJ/cm2. Bantseev et al. have also analyzed the reactive oxygen species and cell viability after UBV irradiation ad 223 and 445 mJ/cm2. In this way, our experimental model of using UBV at 100-400 mJ/cm2 would be appropriate in the evaluation of both the cell death and the production of reactive oxygen species. For these reasons, we believe that the long-term application of carteolol hydrochloride may have a potential to inhibit cataract formation in vivo.
The effect of carteolol hydrochloride as a scavenger of reactive oxygen species is thought to be attributed to its molecular structure. Naito et al. have reported that various synthetic molecules containing 3,4-double bond of quinolone serve as a hydroxy radical scavenger . In fact, rebamipide (2-(4-chlorobenzolamino)-3-[2(1H)-quinolon-4-yl]proppionic acid) exert its anti-ulcer action by scavenging the hydroxyl radicals in gastric mucosa. In addition, Tanito et al. by analyzing the electron spin resonance spin trapping technique have reported that carteolol hydrochloride, which possesses 3,4-double bond of quinolone in its structure, also serves as a hydroxyl radical scavenger .
Although we have seen an inhibitory effect of carteolol hydrochloride on the production of reactive oxygen species after 100, 200, and 400 mJ/cm2, viability of the lens epithelial cells was not increased at 100 mJ/cm2 of UV exposure. This result indicates that cell death after UV exposure is regulated by various factors including intracellular reactive oxygen species and direct damage on DNA and proteins. For this reason, further studies are needed to apply the results of our present study into human. The results of the present study indicated both the increase in the reactive oxygen species and the cell death after UVB irradiation was inhibited by carteolol hydrochloride depends on the conditions. Further investigation should be needed to cleat the protective effect of carteolol hydrochloride after UBV irradiation using in vivo model .
Cataract and glaucoma are major cause of blindness worldwide. This means that cataract surgery is not available in various parts of the world. For this reason, application of eye drops that may inhibit cataract formation would be beneficial for the world health. Although the protective effect of carteolol hydrochloride against UVB induced cataract has not been shown in vivo model, the results of the present study would affect the selection of anti-glaucoma drugs.