Prevention of Lens Epithelial Cell Growth  Using Mibefradil-Containing PLGA Micro Particles

Weidmann, Arne; Kwittner, Sabine; Beck, Ria; Teller, Joachim; Jonas, Ludwig; Nebe, J. Barbara

Prevention of Lens Epithelial Cell Growth in vitro Using Mibefradil-Containing PLGA Micro Particles

Arne Weidmann¹, Sabine Kwittner², Ria Beck², Joachim Teller³, Ludwig Jonas⁴, J. Barbara Nebe^{1
, *}

¹ Biomedical Research Centre, Cell Biology, University of Rostock, Schillingallee 69, D-18057 Rostock, Germany

² Dept. of Ophthalmology, University of Rostock, Doberaner Str. 140, D-18055 Rostock, Germany

³ Micromod Partikeltechnologie GmbH, Friedrich-Barnewitz-Str. 4, D-18119 Rostock, Germany

⁴ Electron Microscopic Centre, Medical Faculty, University of Rostock, Strempelstr. 14, D-18057 Rostock, Germany

Article Information

Identifiers and Pagination:

Year: 2008
Volume: 2
First Page: 112
Last Page: 118
Publisher ID: TOOPHTJ-2-112
DOI: 10.2174/1874364100802010112

Article History:

Received Date: 23/4/2008
Revision Received Date: 22/5/2008
Acceptance Date: 24/5/2008
Electronic publication date: 12/6/2008
Collection year: 2008

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© Weidmann et al.; Licensee Bentham Open

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.

^* Address correspondence to this author at the University of Rostock, Biomedical Research Centre/Cell Biology, Schillingallee 69, D-18057 Rostock, Germany; Tel : + 49 381 494 7771; Fax: +49 381 494 7778; E-mail: barbara.nebe@med.uni-rostock.de

The prevention of the posterior capsule opacification is still unsolved. To interfere with proliferating cells the T-type calcium channel antagonist Mibefradil was immobilized in poly-lactic-co-glycolic-acid micro particles which were fixed at a capsular tension ring and tested in a human organ culture model as well as in human lens cells HLE-B3 in vitro. It is feasible to get a release significantly affecting cell viability and growth evaluated by MTT test and cell cycle analysis. In addition, Bionas^® sensor chips were used for time-dependent adhesion experiments in living lens cells. Interestingly, the concentration of Mibefradil which inhibited subconfluent cells is not effective in confluent cells. This is an important feature for the protection of the intact tissue in the eye.

Keywords: Calcium channel antagonist Mibefradil, lens epithelial cell adhesion, PLGA micro particles, organ culture model.

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Tatsuya Mimura

Department of Ophthalmology
Teikyo University School of Medicine
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RESEARCH ARTICLE

Prevention of Lens Epithelial Cell Growth in vitro Using Mibefradil-Containing PLGA Micro Particles

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