Prevention of Lens Epithelial Cell Growth in vitro Using Mibefradil-Containing PLGA Micro Particles
Arne Weidmann1, Sabine Kwittner2, Ria Beck2, Joachim Teller3, Ludwig Jonas4, J. Barbara Nebe1 , *
Identifiers and Pagination:Year: 2008
First Page: 112
Last Page: 118
Publisher Id: TOOPHTJ-2-112
Article History:Received Date: 23/4/2008
Revision Received Date: 22/5/2008
Acceptance Date: 24/5/2008
Electronic publication date: 12/6/2008
Collection year: 2008
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.
The prevention of the posterior capsule opacification is still unsolved. To interfere with proliferating cells the T-type calcium channel antagonist Mibefradil was immobilized in poly-lactic-co-glycolic-acid micro particles which were fixed at a capsular tension ring and tested in a human organ culture model as well as in human lens cells HLE-B3 in vitro. It is feasible to get a release significantly affecting cell viability and growth evaluated by MTT test and cell cycle analysis. In addition, Bionas® sensor chips were used for time-dependent adhesion experiments in living lens cells. Interestingly, the concentration of Mibefradil which inhibited subconfluent cells is not effective in confluent cells. This is an important feature for the protection of the intact tissue in the eye.